1908.] New Variety of Trypanosomiasis. 547 
began to rise on the evening of February 27, but already, on February 25 
trypanosomes, few but very lively, had been observed in the blood. 
On February 28 they were fairly numerous and at their maximum, since 
from this time up till March 8 they appeared in very few numbers. On 
March 2 the sheath of the penis and the scrotum were much swollen, and 
on March 4 the animal was obviously sick, standing with head down and 
refusing food. By March 7, however, the scrotal swelling was almost 
entirely reduced ; trypanosomes had disappeared from the blood, and the 
animal looked much better. I left by steamer for England on the morning 
of March 8, and am indebted for the subsequent history to members of the 
staff of the Health Department. The improvement noted previous to my 
departure had been merely transient, since emaciation still progressed with 
some fever, and the animal died on March 16. 
At the post-mortem examination solidified yellow cedematous material 
had been. found under the skin. The spleen and liver are said to have been 
congested. 
Donkey.—This animal was injected intravenously with 3 c.c. of the blood 
of the infected horse on February 14. Within the three days subsequent 
to this operation, the temperature was above normal, but this is probably to 
be accounted for by the change of diet and its usual surroundings. From 
this time onward nothing abnormal was observed, nor was anything of the 
nature of fever seen. It was discharged from experiment on March 16, 
when it seemed to be in perfect health. No trypanosomes were seen in its 
blood at any time. 
Dog.—A small pariah dog was inoculated by subcutaneous injection with 
3 cc. of infected blood on February 14. Examinations of its blood were 
frequently made, but no trypanosomes were seen at any period up till the 
time of my departure for England, and the animal was discharged from 
experiment on March 10, when it seemed in perfect health. It has to be 
noted that the inoculation in this case was by subcutaneous injection, and it 
is very possible that infection might have been made had some other means 
of inoculation been used. 
Goat.—A. goat was inoculated by intravenous injection with 3 c.c. of infected 
blood of the horse, on February 14. During the first four days the tempera- 
ture was slightly above the normal, and then subsided. On February 24, 
however, it began to rise, and attained a maximum on March 3, when it had 
reached to 106° F. 
During the subsequent four days it was steadily falling. At no period 
were trypanosomes ever found in the blood, nor did the animal at any stage 
ever show visible signs of any indisposition. 
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