A Bacterial Disease of Stone Fruits 



387 



Fig. 61. — Elberta peach twigs inoculated with pure cultures of Bacterium 

 Pruni. The growth from an eight-days-old-slant Hiss-glucose culture 

 ivas thoroughly mixed in one hundred cubic centimeters of sterile water. 

 Each twig was inoculated at four points by inserting the point of a hypo- 

 dermic needle just below the cuticle and forcing the water containing the 

 organism into the tissues. Inoculations were made on June 25, 1912, 

 and the photograph was taken on August 20, 1912 



a. Check. Sterile water was used 



b and c, Inoculated with a pure culture obtained from an apricot twig 



d, Inoculated with a pure culture obtained from a nectarine txoig 



e, Inoculated with a pure culture obtained from a peach twig 



f, Inoculated with a pure culture obtained from a plum twig 



