400 F. M. Rolfs 



Nutrient agar colonies. — When plantings were made from bouillon 

 cultures six days old at room temperature of about 25° C, the colonics 

 appeared as mere specks in from forty to forty-eight hours. Plantings 

 made from diseased host tissue developed colonies in from three to ten 

 days (usually from four to seven). No growth occurred at 37° C. The 

 colonies were moist and glistening, and at first were almost white but 

 later became amber yellow.' 5 When not crowded, the surface colonies 

 were round and reticulate, and reached a diameter of about one to five 

 millimeters in ten days, with a smooth surface, an entire edge, and a finely 

 granular internal structure. The deeper colonies were elliptical or 

 lenticular. On plates twenty-five days old, colonies measured from ten 

 to twenty-five millimeters in diameter. They had dark, translucent 

 centers surrounded by a much lighter, often concentrically ringed, zone. 



Nutrient agar streaks. — On slanted agar the streak made with a platinum 

 needle developed a moderate amber-white (4) growth. The streak 

 widened slowly, chiefly toward the bottom, made a filiform growth, and was 

 raised, with a slightly contoured surface and an entire edge; it was trans- 

 lucent, with a glistening luster, and was slightly viscid; it did not color the 

 agar on which it grew, at least not for a number of weeks. Growth was mod- 

 erate. After five or six days the growth began to flow downward, and finally 

 accumulated in an amber yellow (1), slimy mass at the base of the slant. 



Nutrient agar stab. — On agar stab the growth was filiform, and was best 

 toward the top of the stab, there being very little or no growth in the 

 lower half of the medium. A circular colony formed on the surface at the 

 point of inoculation, and gradually spread, completely covering the surface 

 in about seven or eight days with a straw yellow (2) growth. The agar 

 was not liquefied nor softened. 



Gelatin colonies. — On gelatin plates at room temperature of about 

 25° C, the colonies appeared as mere specks in from forty-five to fifty 

 hours. They were at first almost white, translucent, moist, and glisten- 

 ing. On the fourth day they measured about one millimeter in diameter. 

 Both buried and surface colonies were round, finely granular, and with an 

 entire edge, becoming with age amber yellow (2) in color. Liquefaction 

 began usually on the third day, the colonies becoming saucer-shaped, 

 spreading, and liquefying the medium in twelve days or more. 



'•' All the color terms used in this paper are based on Rend Oberthilr's Repertoire de Couleurs, published 

 by La Soci6td Francaise des ChrysanthSmistes in 1!").") 



