406 F. M. Rolfs 



Five days after inoculation a cloudiness appeared in the open arm of 

 each of the inoculated tubes. This turbidity increased with age, extend- 

 ing more than halfway into the U in the dextrose, saccharose, and maltose 

 cultures; but at the end of twenty-five days there was no growth in the 

 closed arm and no gas. There was a well-defined line between the growth 

 in the bulb and the clear liquid in the closed arm, which seemed to point 

 strongly toward the aerobic nature of the organism. A pellicle had 

 formed on all the dextrose, maltose, and saccharose cultures, and in 

 most cases there was a yellow, slimy growth of the organism at the bottom 

 of the open arm. There was very little growth in the mannite tubes. 



The cultures were tested for acidity after they had grown for twenty- 

 five days. In glycerin the acidity increased from + 0.8 to + 1 ; in 

 saccharose, from +0.9 to 4-1.1; in mannite, from +0.9 to -f- 1; in 

 dextrose, from + 1 to + 2.5; in maltose, from + 0.8 to + 1; in lactose, 

 from + 0.8 to +1.1. 



Uschinsky's solution. — In the test with Uschinsky's solution, tubes 

 were inoculated from a four-days-old broth culture. A one-millimeter 

 loop of the material was placed in each tube. 



The tubes were all clouded after three days. The solution often became 

 viscid in about twenty-five days. A cream yellow (1) pellicle formed in 

 from twenty to thirty days or later. The pellicle finally settled, forming 

 a slimy, cream yellow (3) bacterial mass in the bottom of the tube. 



Sodium chloride bouillon . — A one-millimeter loop of a six-days-old, 

 + 12, peptonized beef broth culture was placed in tubes of peptonized 

 beef broth containing, respectively, 1, 2, 3, 4, 5, 6, and 7 per cent of chemi- 

 cally pure sodium chloride. Three tubes were inoculated with cultures 

 of each kind of host ■ — apricot, nectarine, peach, and plum. At the end 

 of seven days clouding was apparent in all tubes containing 1, 2, and 3 per 

 cent of sodium chloride. Both the peach and the plum organisms grew 

 in a 3.5-per-cent salt bouillon. There was no clouding in the 4-, 5-, 6-, 

 or 7-per-cent solutions. 



Growth in bouillon over chloroform. — By means of a sterile pipette, five 

 cubic centimeters of chloroform was added to fifteen cubic centimeters of 

 bouillon. Fifteen tubes were shaken twice a day during the period of this 

 test. On the eighth day the tubes were divided into five lots, four of which 

 were inoculated with six-days-old bouillon cultures of apricot, nectarine, 

 peach, and' plum organisms, respectively; the fifth lot being used as a 



