Bean Anthracnose 



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sterilized bean pods were placed in 4 liters of tap water and the spores 

 were washed off. The suspension was then filtered through a mat of 

 absorbent cotton to eliminate fragments of plant tissue. Twenty-one 

 pots of soil were inoculated by pouring over the surface of each about an 

 equal portion of the suspension. There being some of the suspension 

 left over from the first application, a double portion was applied to three 

 pots. Four pots did not receive any spores, and these were labeled Checks. 

 Several thicknesses of cheesecloth were tied over the top of each pot and 

 the pots were placed outdoors. In the course of the winter and spring, 

 the pots were taken into the greenhouse and planted with Davis White 

 Wax, a susceptible variety, the seed used having come from healthy pods 

 and having been carefully sorted. Twenty-five seeds were planted in 

 each pot. After the beans were planted, unused sphagnum was placed 

 over the surface to the depth of an inch to keep the surface of the soil 

 in a moist condition. At the last trial, on June 8, four of the pots were 

 inoculated again with a fresh suspension of spores as a control. The 

 results are given in table 3. It was thought that the pots planted on 



TABLE 3. Results Obtained by Growing Beans in Soil That Had Been Inoculated 

 with a Suspension of Spores of Colletotrichum lindemuthianum, as Compared 

 with Those Grown in Uninoculated Soil 



