Wire Stem of Cabbage 



21 



added to the soil immediately surrounding the seed piece and a little 

 above the upper surface of the tuber, so that any sprouts would pass 

 directly through it. The pot was filled with the remaining soil. All 

 the pots were kept in the same greenhouse where the previous experiment 

 had been conducted. This house was comparatively cool, having a 

 temperature of approximately 8° to 15° C. at night, and about 10° to 18° C. 

 during the day. The soil was kept moderately moist, but no attempt was 

 made to control accurately the moisture content. All the tubers sprouted. 

 When they were from two to three inches high, many were removed from 

 the soil and examined, but no lesions were observed on the stems. The 

 plants were carefully replaced and allowed to grow for two and one-half 

 months. At the end of that time they were removed from the soil, and 

 the stems and rootlets were examined for lesions, and the new tubers and 

 the seed pieces for sclerotia. 



Parallel with this experiment, fifty tubers were placed in soil infected 

 with the cabbage strain of the fungus. When the sprouts were two or 

 three inches high, and no lesions were developing, ten plants were re- 

 inoculated just beneath the surface of the ground and these were kept 

 in a moist chamber for forty-eight hours. They were then removed 

 to the greenhouse bench. A little later, when the plants were from six 

 to eight inches high, cabbage seed was sown around the base of twenty- 

 five of Hie plants growing in the soil infected with the fungus from cabbage. 



