27 



Pink Salmon Fry- 

 Respiration rates of newly emerged pink salmon fry from 

 Sweltzer Creek incubation channel were measured at low concentrations 

 of tetrachlorocatechol in an attempt to establish the minimum 

 concentration at which tetrachlorocatechol influenced their 

 respiration. Relative respiration rates varied at 0.175, 0,150 

 and 0.100 ppm tetrachlorocatechol. Results from Trials P and S 

 shov/ed a general trend tov;ard increasing relative respiration rate 

 with greater tetrachlorocatechol concentraticns but data from 

 Trials and R failed to confirm such a trend (TABLL 8 and FIGURE 4). 

 The difference may have been caused by the fact that fry in Trials 

 and R were held overnight in tetrachlorocatechol while fry in 

 Trials P and S were not, but it is Delitived more likely that 

 variations in fry activity were a primary cause of the variable 

 results. Observations of pink salmon fry under conditions similar 

 to those in the trials, except that light was not excluded, indicated 

 that fry exhibited a high state of activity which was influencea 

 primarily by behavior of individual fish rather than concentration 

 of tetrachlorocatechol. ijxcitability during confinement may have 

 been related to the fact- that the fry were ready to migrate to the 

 Fraser Rivur estuary. 



Although the data are not precise, results in FIGURE 4 suggest 

 that tetrachlorocatechol caused an increase in respiration rate at 

 concentrations greater than 0,10 ppm. It appears that respiration 

 rate was unaffected at 0,05 ppm tetrachlorocatechol. 



Biological Treatment for Oxidation 

 of Chlorinated Catechols 



A test of the ability of activated sludge to oxidize 

 tetrachlorocatechol added to neutralized bleach waste (iffiW) was 

 conducted and a 96-hr bioassay used to evaluate treatment efficiency, 

 ^fter 24 hr of activated sludge treatment, neutralized bleach waste 



