Reagents 



Di- aiiJ tetrachlorocatechol were supplied through courtesy of 

 Dr. K.V. Sarkanen, Institute of Forest Products, University of 

 Washington. Tetrachlorophenol was i;;astman Techriical Grade while 

 2,5-dichlorophenol was Eastman Grade. 



Tests of Acute Toxicity 



Acute (lethal) toxicity of di- and tetrachlorocatechol to young 

 pinlc and sockeye salmon v;as measured in 96-hr and 31-day bioassays. 

 In addition, the acute toxicities of di- ana tetrachlorophenol to 

 sockeye fry during 96-hr exposures were determined for comparison 

 with toxicity of the chlorinated catechols. Bioassays using bleach 

 waste were of 96-hr duration. 



Ninety-six Hour Exposure 



Short-term exposure (96 hr) of fish to toxic materials wa? 

 carried out in standing-water bioassays using plastic aquaria of 

 1- to IG-liter capacity. Five to 10 fish were used in each test 

 and concentrations of fish ranged from 0.3 to 3.9 gm per liter 

 (TAisLE 1). Gultus Lake water was used ana dissolved oxygen was 

 kept near saturation with compressed air. 



TABLb 1 - Concentrations of fish in short-term bioassays. 



