TAULE OF COWTMTS 



PAGE 



INTRODUCTION 1 



ORIGIN AND POTENTIAL TOXIC ACTION OF CHLORINATED 



CATECHOLS , . . . . 3 



MTERIALS AND METHODS 6 



Sources of Fish 6 



Reagents 7 



Tests of Acute Toxicity 7 



Ninety-six Hour Exposiire 7 



Thirty-one Day Exposure 8 



Measurements of Respiration Rate 9 



Biological Treatment of Bleach Waste Containing 



Added Chlorinated Catechol 11 



RESULTS 12 



Acute Toxicity of Chlorinated Catechols 12 



Ninety-six Hoiir Exposure 12 



Thirty-one Day Exposure 15 



Acute Toxicity of Chlorinated Phenols 15 



Condition and Behavior of Test Fish 17 



Effect of Chlorinated Catechols on Respiration . . 17 



Sockeye Salmon Alevins 17 



Sockeye Salmon Fry 22 



Pinl-c Salmon Alevins 24 



Pinlc Salmon Fry 27 



Biological Treatment for Oxidation of Chlorinated 



Catechols ........ 27 



DISCUSSION 30 



Acute Toxicity 30 



Ninety-six Hour Exposure ..... 30 



Thirty-one Day Exposure 32 



Relative Toxicity and Effect of Chlorine 



and Hydroxyl Substitution 33 



Sublethal Toxicity ox Chlorinated Catechols .... 35 



Biological Treatment and Chlorinated Phenolic 



Compounds in Bleach Waste 37 



Potential for Toxic Conditions 39 



CONCLUSIONS 40 



LITERATURE CITED . 42 



