29 



onto artificial diet. The technique is equally adaptable to maintenance 

 of small cultures and to mass-rearing programs. Eggs were collected from 

 cotton squares (flover buds) by chopping the squares in water with a house- 

 hold food chopper. The eggs were removed, surface sterilized, and submerged 

 in sterile water in a sterile petri dish. The egg-water suspension was drawn 

 into a disposable bacteriological capillary tube and ejected onto the scratched 

 surface of larval diet in petri dishes. The petri dishes containing the eggs 

 and diet were held 14-17 days at 26°C for development, after which the adults 

 were removed and held in cartons for feeding and egg production. The moisture 

 content of diet before eggs were implanted was the most important of the con- 

 ditions Investigated in determining the percentage of adults that were obtained. 



52. , and Lambremont, E. -l. 1967. Changes in stored glycogen and lipids 

 associated with diapause of the adult boll weevil, Anthonomus grand is 

 (Coleoptera: Cure ilionidae) . Ann. Entomol. Soc. Am. 60: 866-868. 



Increases in glycogen and lipids are known to take place as newly emerged adult 

 boll weevils feed and undergo diapause development. This process is generally 

 assumed to represent storage of important nutrient reserves which are drawn on 

 when feeding is no longer possible during the overwintering period of dia- 

 pause. The present communication reports the results of some preliminary, 

 experiments made to determine whether the adult weevil prefesentially depletes 

 its carbohydrate reserves before degrading its lipids or whether both decrease 

 simultaneously. 



53. ; Nettles, W. C, Jr.; and Novak, A. F. 1968. Physicochemical 

 characteristics of glycogen from Anthonomus grandis . Comp. Biochem. 

 Physiol. 24: 163-175. 



Glycogen was extracted from the boll weevil, Anthonomus grandis Boheman, with 

 5% trichloroacetic acid (TCA) and with cold chlorofonnglyclne buffer (cold 



\ 



