r ■ ' ' / // 



30 



water extraction). Glucose was thp only sugar detected in acid hydrolyxates . 

 The average chain lengths, as determined by methylation, hydrolysis, silaniza- 

 tion and gas-liquid chromatography, were 11.0, 11.9, and 11.8 glucose units, 

 respectively, for glycogen extracted with cold water from eggs and larvae and 



I glycogen extracted with TCA from larvae. The high percentage (82) of 2,3,6- 



% 



J 



I trimethylglucopyranose obtained indicated that the principal linkage between 



I glucose units was 1,4. Similar i.r. spectra were obtained from glycogens ex- 



j tracted with TCA and cold water. Boll weevil glycogen complexes with iodine- 



e iodide gave absorption spectra similar to a rabbit liver glycogen standard. 



The optical rotation of glycogen extracted with TCA from larvae was +200*0". 



Measurements of optical rotation could not be made of material extracted with 



cold water because of high opalescence. 



54. Biggers, C. J., and Bancroft, H. R. 1976. Inheritance of hemolymph 

 esterases of the cotton bol] weevil ( Anthonomus grandif ? Boh.). J. Tenn. 

 Acad. Sci. 51: 59. 



Four esterase regions from boll weevil hemolymph were demonsi'.rated by vertical 

 polyacrylamide gel electrophoresis. They were designated Esterases I, II, III, 

 and IV in order of anodal migration. The Inheritance pattern for Esterase II 

 was determined by a series of laboratory matings. The evidence suggests that 

 the esterases in this region are controlled by a pair of autosomal codominant 

 alleles. 



55, , and Bancroft, H. R. 1977. Esterases of laboratory-reared and field- 

 collected cotton boll weevils, Anthonomus grandis Boh. : polymorphism of 

 adult esterases and formal genetics of Esterase II. Biochem. Genet. 

 15: 227-233. 



. -si.-'S.-J'^i 



