23 



dead by the end of the 2nd week. Thus, irradiated boll weevils may be effec- 

 tive ii; a program involving the release of sterile insects, but the releases 

 would hive to be made at least weekly and preferably every 5 days. 



39. ; Mattix, E. B.; and Wilson, N. M. 1968. Multiple matings and use of 

 sp^rm in the boll weevil, Anthonomus grand Is . Ann. Entomol. Soc. Am. 

 61: 11A8-1155. 



Virgin female boll weevils, Anthonomus grand is Boheman, from a mutant strain 

 with pearl-colored eyes were mated in various combinations to males of the 

 same pearl strain or to males with brown (wild-type) eyes to study the use 

 of sperm in multiple matings. Sperm from the last mating took precedence 

 over sperm from other matings only when at least 24 hours separated the 

 matings. The advantage of sperm from the last mating ranged from about 10% 

 when 1 day separated matings to 90% when 4 days intervened. This advantage 

 lasted from about 7 days when the interval between matings was short to more 

 than 45 days from the longest interval. It is concluded from other data that 

 most field females will follow the pattern of sperm use shown by closely spaced 

 multiple matings. 



40. , and Mitlin, N. 1967. Developmental and reproductive effects of 

 heavy water (D2O) in the diet of the boll weevil. J. Econ. Entomol. 

 60: ^47-655. 



The substitution of various concentrations of heavy water (D2O in place of H-O) 

 In the adult and larval diets of the boll weevil, Anthonomus grand is Boheman, 

 showed that: (1) levels of heavy water above 60% of the total water incorporated 

 into the adult diet reduce egg production in the treated fetimles, but the action 

 is quickly reversible when normal food is restored; fertility in treated indivi- 

 duals is not affected; (2) as the percentage of heavy water is increased in 

 the larval diet (a) the percentage of eggs developing in the treated diet 



