62 



American cockroach ( Periplenata amerlcana (Linnaeus)), bollworm (He liothis zea 

 (Boddie)), and tobacco budworm ( Heliothis virescens (Fabricius)) and white 

 rats. However, formation of the toxic unsubstituted amide derivative of 

 Azodrin by complete oxidative N-demethylation apparently was a minor re- 

 action. Similar metabolites of Azodrin were formed in the different test 

 animals, but at widely different rates. 



108. , and Lindquist, D. A. 1968. Cholinesterase in boll weevils, 

 Anthonomus grandis Boheman. I. Distribution and some properties of 

 the crude enzyme. Comp. Biochem. Physiol. 25: 639-649.. 



1. Homogenates of adult boll weevil, Anthonomus grandis Boheman, apparently 

 contained only one enzyme that catalyzed hydrolysis of choline, esters. 2. 

 The enzyme was primarily associated with the particulate fraction of homo- 

 genates and was almost evenly distrbutcd between the abdomen (55 percent) 

 and the head plus thorax (45 percent) . 3. Based on information obtained 

 from studies of substrate specificity, esterine inhibition, and influence 

 of substrate concentration, pH and temperature on activity, the enzyme was 

 tentatively classified as an acetylcholinesterase (AChE) . 4. In adult weevils 

 of a colony reared in continuous light, the levels of AChE were relatively 

 constant during a 24-hr period; however, when the weevils were reared with a 

 14L-10D daily photoperiodic regimen, enzyme activity definitely was higher 

 in weevils during the light hours. 



109. ; Lindquist, D. A.; and Coppedge, J. R. 1967. Metabolism of 2-methyl- 

 2-(methylthio)propionaldehyde 0-(methylcarbamoyl)oxime (Temik, UC-21149) 

 in insects. J. Agric. Food Chem. 15: 610-616. 



The fate of radiolabeled 2-methyl-2-(methylthio)propionaldehyde O-(methylcarbamoyl) 

 oxime (Temik, lIC-21149) in insects was examined. Topically applied Temik was 

 absorbed rapidly by adult boll weevils, Anthonomus grandis Boheman, but very 



