80 



pollen meal. Weevils collected from the field must be preserved in ethanol 

 {7bX) to preserve the pollen grains intact in the gut. A technique for ex- 

 traction and slide mounting of pollen grains is 'given. 



146. Chambers, D. L. 1977. Quality control in mass rearing. Annu. Rev. 

 Entomol. 22: 289-308. 



Great progress has been made in recent years in understanding the effective- 

 ness of a massive insect release program. Techniques have been developed to 

 test most of these; routine monitoring is now conducted on some. Protocols 

 exist for assessing quality and continuity of production. Resources need to 

 be applied to understanding and incorporating behavioral requisitic into 

 monitoring protocols that realistically reflect program objectives. 



147. Chang, S. C, and Stokes, J. B. 1979. Conjugation: the major metabolic 



14 

 pathway of C-dif lubenzuron in the boll weevil. J. Econ. Entomol. 



72: 15-19. 



14 

 The metabolic fate of C-dif lubenzuron was studied after it was injected into 



14 

 Anthonomus grandis grandis Boheman. Four days posttreatment , recovery of C 



was 57.7% from treated weevil; and 25% from the excreta. In the weevil, 57.3% 



14 

 of the C were recovered as unchanged dif lubenzuron while 0.4% was recovered 



as conjugates. In the excreta, ca. 2% each of diflubenzuron and 2, 6 ;^^c^..„^^. 



benzamide were found and the remainder was conjugates. The total amount of 



conjugates was ca. 18.7%. Percentages were calculated on the basis of the total 



injected amount. The conjugates consist of 3 or more compounds, all of \«?nich 



were immediately excreted as very polar compounds. Upon acid hydrolysis, 



radioactive moieties from the conjugates were identified as N[ [ (4-chloro-2- 



hydroxyphenyl)amino] carbonyl]-2, 6-dif luorobenzamide and N-[ [ (4-chlorophenyl) 



amino]carbonyl]-2, 6-dif luoro-3-hydroxybenzamide, plus 2 unknowns. 



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