86 



of larval cultures). Attempts to prevent contamination have included: 

 sterilizing the diet, incorporating the antibiotic tetracycline (Polyotic®, 

 0.2% final diet concentration), spraying the surface of the diet with a 6% 

 solution of formaldehyde, and partially desiccating the diet after implanta- 

 tion of the eggs. However, these preventive measures have been ineffective 

 in controlling a sline-producing contaminant which is evidently widespread 

 in many types of insect cultures. The purpose of this research was to 

 identify the contaminant and to determine a means of controlling it with 

 minimal amounts of antimicrobial additives. 



159. Cleveland, T. C, and Parencia, C. R. 1976. History of the USDA Cotton 

 Insects Research Laboratory, Tallulah, Louisiana, 1909-1973. Bull. 

 Entomol. Soc. Am. 69: 403-407. 



On June 30, 1973, after 64 years of continuous operation, the USDA Cotton 

 Insects Research Laboratory, Tallulah, Louisiana, closed. Since the Labo- 

 ratory began in 1909, many prominent entomologists have been members of 

 the staff. Their pioneering efforts in evaluating and developing chemical 

 insecticides and application techniques for the control of pest insects in 

 cotton are widely recognized. The Laboratory was the acknowledged leader 

 in research on the boll weevil, Anthonomus grand is Boheman, for over half 

 a century and provided an invaluable service tc cotton growers when the 

 boll weevil invaded the Mississippi De] ca and spread to the East Coast. 



160. , and Scott, W. P. 1967. An improved technique for aerial applica- 

 tions of ultra-low-volume materials to experimental plots. J. Econ. 

 Entomol. 60: 1761-1762. 



An improved system is described in detail. Some advantages of the new system 

 are: (1) constant pressure is maintained; (2) the spray is turned on and off 

 instantly; (3) calibration is accurate; (4) pump priming is eliminated; (5) 



