122 



days and in males given small increments of irradiation (fractionated irradiation) 

 during the late pupal and early adult stages. Both groups produced much less 

 pheromone during the next 3 days. The acute irradiated weevils, destined to 

 die soon, produced no pheromone after the 9th day, whereas the fractionally 

 irradiated males began to show a partial recovery. Males fractionally irradiated 

 entirely during the adult stage produced a moderate but constant amount of phero- 

 mone during the entire 12-day period. Measurements of attractiveness of treated 

 males placed in field traps produced results that were in general agreement with 

 the laboratory data. Male sterility for all 3 treatments was ca. 99%, and from 

 66-82% of the males were able to transfer speirm 7 days after treatment. Females 

 irradiated during the pupal and adult stages were completely sterile, producing 

 no eggs. The 2 groups of females irradiated as adults produced some eggs and it 

 was necessary to incorporate a dip treatment consisting of 0.02% diflubenzuron 

 (Dimilin®; N-[ [4-chlorophenyl)animo]carboynl]-2,6-ditluorobenzamide) in acetone 

 to suppress viability. 



228. ; Slatten, B.; and Burks, M. L., Jr. 1967. Essential fatty acids in 

 the diet of the boll weevil, Anthonomus grandi s Boheman (Coleoptera: 

 Curculionidae) J. Insect Physiol. 13: 187-200. 

 Larvae of the boll weevil ( Anthonomus grandis Boheman) developed normally in 

 diets without added fatty acids. However, the adults from the fat-deficient 

 diets laid fewer eggs than normal. Egg production was highest when the diets of 

 the larvae and adults of both sexes contained a mixture of triglycerides that 

 included trilinolein or trilinolenin. Omission of triglycerides from the diet 

 of the larvae influenced fecundity more than their omission from the diet of the 

 adults. Symptoms of fat deficiency in adults were most pronounced when they 

 were reared from eggs that were obtained from parents being fed with a diet 

 low in fat. Gas chromatographic analyses of these eggs showed that they con- 





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