182 



monitored. The monitoring activities were concentrated in the following 

 three areas: (1) Impact on cottonf ield-inhabiting nontarget insect species, 

 including both insect pest species and natural enemies; (2) impact on honey 

 bees frequenting cottonfields from beehives in the vicinity; and (3) impact 

 on nontarget wildlife species. 



351. Harwalkar, M. R.; Haynes, J. W.; and Mitlin, N. 197A. A quick staining 

 procedure to determine the testicular damage to boll weevils sterilized 

 ■ with busulfan. Entomol. Exp. Appl. 17: 319-321. 

 The region surrounding the gennarium of a normal untreated testes is intensely 

 stained and appears as a discrete band in the middle region. This region is 

 constituted of spermatocytes in various stages of development. In the treated 

 testes, this region has disintegrated, since the identity of the discrete band 

 is lost, probably because of the disappearance of the spermatocytes. All areas 

 of treated testes took less stain than untreated testes, an of the spermatocytes. 

 All areas of treated testes took less stain than untreated testes, an indication 

 that fewer germ cells were present. However, the germarial area of treated 

 testes was more densely stained than other areas. Evidently the sperm below 

 the germarium take up the stain and the germarium appears more intact. We 

 surmise that the granular structures in the germarium region in the treated testes 

 are the disintegration products of germarial and spermatocyte cells. However, 

 their exact nature could not be determined. The differences in staining pattern 

 between untreated and treated testes were clear, and we had no difficulty in 

 assessing them. However, we could not precisely correlate the degree of 

 sterility of substerilized weevils (those fed for 1-5 days) with the results 

 of the staining procedure. Thus tVie testes from males fed busulfan 1 or 2 

 days were comparatively little damaged and the staining pattern was almost 

 normal. Weevils fed 3 days had more damage (alveit variable) and intermediate 

 or incomplete sterility, and the staining pattern resembled neither completely 



. . -1. 



