189 



366. ; Mitlin, N.; Sloan, C. E. ; and Dawson, J. R. 1973. Busulfan: 

 development of Improved methods of sterilizing boll weevils. J. Econ. 

 Entomol. 66: 619-622. .■■._.,..;.'. 



The standard method of sterilizing Anthonomus grandis Boheman (6-day feeding of 

 0.1% busulfan, 1-4-butanediol dimethanesulfonate) adequately sterilizes males but 

 does not. induce a high degree of sterility in females, and mortality is less than 

 40% after 2 weeks. Feeding higher concentrations of busulfan alone or lower con- 

 centrations of busu fan in combinations with methotrexate, hempa, or apholate 

 for 6 days or less or feeding 0.1-0.2% busulfan followed by low doses of gamma 

 radiation induces • . tually complete sterility in both sexes but causes fairly 

 high mortality. A 4-day feeding of 0.2% busulfan + 0.25% methotrexate resulted 

 in complete sterility in females and almost complete sterility in males, and 

 mortality was less than 40% by 3. weeks after treatment. 



367. , and Wright, J. E. 1979. Fumigation of boll weevil pupae with 

 bisazir to induce sterility. J. Econ. Entomol. 72: 823-825. 



When pupae of Anthonomus grandis grandi s Boheman were fumigated for 5 h in a 

 circulating, saturated atmosphere of bisazir, 99% of the males were steri- 

 lized, and egg laying by treated females outcrossed with normal weevils was 

 reduced by 90.6%. Sterile males transferred 11.8% more sperm than control 

 males the 1st week posttreatment but 42.6% less the 2nd week. 



368. ; Wright, J. E.; Davich, T. B.; Roberson, J.; Griffin, J. G.; 



and Darden, E. 1978. Boll weevil: experimental sterilization of large 

 numbers by fractionated irradiation. J. Econ. Entomol. 71: 943-946. 

 Boll weevils, Anthonomus grandis grandis Boheman, 9 days after egg implantation 

 in the larval diet were transported from the Boll Weevil Research Laboratory, 

 Mississippi State, MS, to the Comparative Animal Research Laboratory, Oak Ridge, 

 TN, and irradiated with 6.9 krad (test 1) or 7.2 krad (test 2) of Co gamma 



