191 



to 12% more females than 7-day-old males exposed to the acute treatment. 



371. Hedin, P. A. 1969. Chemical components in the cotton plant and their 

 effect on boll weevil behavior. JLn Insect-Plant Interactions; Report 



of a Work Conference, pp. 29-30. National Academy of Sciences, Washington. 

 A summary of work from 1962-1969 wus presented on chemical components iaolated 

 from the cotton plan;, Gossypium hirsutum L., and their effect on boll weevil 

 ( Anthonomus gnandis Boh.) behavior. Feeding stimulations and attractancy v;ere 

 found to be elicited by a profile of plant compound, not individuals. The 

 identity of the components was reported. 



372. . 1976. Grandlure development. ^ Boll Weevil Suppression, Management, 



and Elimination Technology. Proceedings of a Conference, February 13-15, 197A, 

 Memphis, Tennessee. U.S. Agric. Res. Serv. [Rep.] ARS-S-71, pp. 31-33. 

 In the initial chemical work, extractions of males with ilchclororaethane pro- 

 duced a substance that was consistently attractive to females, but a siinilar 

 extract of mixed insects was unattractive to females. When the male extract 

 was steam-distilled, the solvent extract of .the distillate proved to have 

 greater attractiveness for females than any of the other extracts that had 

 previously been prepared. Assays of the steam distillate in concentrations 

 as low as one male equivalent, and simultaneous assays of live males, indi- 

 cated that approximate quantitative removal of the attractant had been ob- 

 tained. When the frass of males, and later, mixed sexes was steam-distilled, 

 it also was highly attractive to males (Tumlinson ct al. 1968). Because the 

 trass could be collected from the mass-rearing facility, which was primarily 

 be.ing used for the production of sterile males, over 50 kg was eventually ob- 



However, it is estimated that 6 million additional insects were used for this 

 work. Our next step was to fractionate the distilled extract on a silica gel 



I 



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talned and processed for isolational work at only a n.^minal direct cost. |; 



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