198 



384. ; Miles, L. R. ; Thompson, A. C; and Gueldner, R. C. 1972. Consti- 

 tuents of the boll weevil. VI. Effect of free fatty acid content on 

 larval development. J. Econ. Entomol. 65: 1284-1286. 



When isolated free fatty acids (FFA) and methyl ester fatty acids (MEFA) 

 from larvae of Anthonomus grandis Boheraan were applied topically to larvae 

 and pupae, juvenile hormonelike characters such as retardation, arrest, 

 formation of intermediates, and deformities occurred. Effective doses of 

 both standard and isolated unsaturated FFA and MEFA were (EDcq) 25-30ug 

 for larvae and 15-20 yg for pupae. The FFA in larvae does not exceed 

 9 ug. The endogenous FFA levels drop significantly during larval develop- 

 ment, reaching a minimum just before the onset of pupation. 



385. ; Miles, L. R. ; Thompson, A. C. ; and Minyard, J. P. 1968. Constitu- 

 ents of a cotton bud. Formulation of a boll weevil feeding stimulant 

 mixture. J. Agric. Food Chera. 16: 505-513. 



Highly active feeding (puncturing) stimulant mixtures were formulated for 

 the boll weevil ( Anthonomus grandis Bohenan) from known cotton constituents, 

 common metabolites, and compounds inducing primary mammalian sensations 

 of taste and odor. Of 286 compounds bioassayed individually, 52 elicited 

 substantial activity, and 14 of these had 'pt-eviously been reported in _ 

 ^utc onr The incsect was found to express preference for sweet, a^ur, and 

 cooling taste properties, but odor preferences were diff^cult to establish. 

 Compatability of the individually active components were necessary for formu- 

 lation of active mixtures. An 8-coroponent mixture of 3-sitosterol, 15 pentade- 

 canolide (15-hydroxypentadecanoic acid ^-lactone) , 1,8-cineole (l,8-epoxy-£- 

 methane), N,N-dimethylaniline, vanillin, mannitol, rhamnose, and 0.1 M phos- 

 phate buffer, pK 7.0, was superior to cottonseed oil and competitive with 

 aqueous bud extract baits. 



