260 



By comparison of the radiorespirometric formation of ''" C0„ of adults injected 

 with differentially labeled glucose and pyruvate to the specific activity of 

 lipid . ynthesized from these substrates, it was determined that glycolysis is 

 the principal pathway by which acetate is produced. The patterns suggested 

 that the boll weevil possesses the pentose phosphate pathway, which also 

 accounts for a significant oxidation of glucose. Recombinations of labeled 

 carbon at "is within and between intermediates of the pentose cycle allow syn- 

 thesis of labeled fatty acid to take place with those carbons of glucose 

 which could not ordinarily enter fat synthesis by glycolysis alone. 



A95. ; Bennett, A. F.; and Stanley, R. F. 1967. Modified laboratory tech- 

 niques for preparing sterile larval diets for the boll weevil. J. Econ. 

 Entomol. 60: 1473. 

 By autoclaving the whole diet minus the B-vitamins, we have returned to suc- 

 cessful rearing of boll weevils in both vials and petri dishes. Also this diet, 

 which is completely sterile, can he used to study lipid synthesis in the boll 

 weevil because C^ -labeled metabolites can be added directly. (Obviously any 

 possible effects of microbial contaminants in such type of work must be ex- 

 cluded). Although we have autoclaves C fatty-acid diets with no detectable 

 loss of radioactivity, we recommend filtering them through a Millipore filter 

 into the cooled diet. Aliphatic and aromatic hydrocarbon solutions of such 

 labeled metabolites will not damage the filter. 



496. ; Bumgarner, J. E.; and Bennett, A. F. 1906. Lipid biosynthesis in 



the boll weevil ( Anthonomus grandis Boheman) (Coleoptera: Curculionidae) : 

 distribution of radioactivity in the principal lipid classes synthesized 

 from C^^-1-acetate. Comp. Biochem. Physiol. 19: 417-429. 

 1. The boll weevil, Anthonomus g rand is Boheman, synthesized fatty acids and 

 hydrocarbons from acetate. The fatty acids were rapidly esterified with glycerol 



