261 



and Lterol or were incorporated into the phospholipid fraction. Radioactivity 

 was not incorporated into the sterol fraction, an indication of the absence of 

 sterol biosynthesis from acetate. 2. The feeding history and developmental state 

 of the adult boll weevil determined the eventual location of the newly synthe- 

 sized .fatty acids. Newly emerged unfed adults incorporated the fatty acids 

 principally into the phospholipids. In insects injected with radiolabeled 

 acetate after several days of feeding, the new fatty acids were distributed 

 to the glycerides. 3. Larvae fed a diet containing labeled acetate had an 

 entirely different labeling pattern with much higher amounts of C appearing 

 in the free fatty a^id fraction. 



497. , and Wood, R. 1968. Glyceryl ethers in insects: identification of 

 alkyl and alk-1-enyl glyceryl ether phospholipids. Lipids 3: 503-510. 

 Alkyl and alk-1-enyl ethers have been identified in the phospholipids of three 

 insect species, the American cockroach ( Periplaneta americana ) , the tobacco 

 budworm ( Heliothis virescens ), and the boll weevil ( Anthonomus grandis ). 

 Glyceryl ethers were not detected in the neutral lipids. The ethers were found 

 in the phospholipid fraction of whole insects and in isolated fat body tissue. 

 The ether content varied among the three insect species, and fluctuated during 

 various developmental stages. Gas liquid chromatographic analysis of the alkyl 

 glyceryl*"ethers and aldehydes derived from the alk-1-enyl glyceryl ethers of 

 the cockroach and budworm showed striking differences in chain length. However, 



the hydrocarbon side-chain of the two ether fractions were similar in length 



14 

 for each species. Preliminary evidence indicates that 1- C-acetate can be 



incorporated into alkyl ethers but not into alk-1-enyl ethers of Heliothis 



pupae. 



