304 



592. ; MLtlin, N.; Haynes, J. H.; and Davich, T. B. 1972. Feeding stimu- 

 lants added to diet adult boll weevils. J. Econ. Entomol. 65: 1190. 



In studies to determine whether additives or other ingredients would increase 

 the ingestion of the adult boll weevil diet used at this laboratory, several 

 additives increased initial ingestion but total ingestion was not affected. 



593. McLaughlin, R. E. 1966. Infection of the boll weevil with I^ttesia grand is 

 induced by a feeding stimulant. J. Econ. Entomol. 59: 909-911. 



Spores of Mattesia grandis McLaughlin were applied in a feeding stimulant formu- 

 lation to cotton plants to induce boll weevils, Anthonomus grandis Boheman, to 

 Ingest infective doses. Water suspensions of spores without the stimulant in- 

 fected only 1 of 162 weevils in a field test. Formulation in the form of viscous 

 liquid and granules that contained sugar, agar, feeding-stimulant extracts from 

 cotton squares, and spores were tested in 30X30X30- inch field cages; disease 

 incidence ranged from 18.3 to 93.3%. In 6X7X24-foot cages, 61. A% of the adults 

 were diseased 10 days after the last application. In 1/8-acre cages, numbers 

 of boll weevils on the treated plants were reduced significantly by the di- 

 sease. The principle that materials which elicit a behavioral response may 

 be employed to Induce host insects to acquire infective doses of pathogens 

 was establish^. 



594. . 1966. Laboratory techniques for rearing disease-free insect colonies: 

 elimination of Mattesia grandis McLaughlin, and Nosema sp. from colonies 

 of boll weevils. J. Econ. Entomol. 59: 401-404. 



Mattesia grandis McLaughlin and a microsporidian, Nosema ep., were eliminated 

 from laboratory colonies of the boll weevil, Anthonomus grandis Boheman, by a 

 system of "Unit Integrity" that is adaptable to rearing procedures for diffe- 

 rent species of Insects, makes best use of available labor and facilities, and 

 permits production of the largest possible disease-free colony per period of 



