■,^-^;. ^y,..^,uuJ .■ ^ ^> . ■■^e ^Mw^^.ln::». l■^■ : ■■ ' ■ ■ - i f y in' jTyy* ' "*!;: ' - ■ -Mr-" ' ^» ' '' '' M^ :' ^ ' "." ;' V .' ^'^' * "'-T -'-' " " .^ 



306 



as many adults as the check population. The treated adult population remained 

 stabilized; the check had sharply defined, eruptive increases in number of adults 

 as each generation emerged from squares or bolls. A feeding stimulant with spores 

 or other effective agents might be effective in reducing populations in large open 

 fields. 



596. . 1969. Glugea gasti sp. n., a microsporidan pathogen of the boll 



weevil Anthonomus grandis . J. Protozool. 16: 84-92. 

 Glugea gasti sp. n., a microsporidan pathogen of Anthonomus grandis Boheman (the 

 boll weevil), is described and a probable life cycle presented. The alimentary 

 canal, and probably the mesenteron 1st, is the initial site of infection, although 

 the disease later becomes generalized throughout most body tissues. Binucleate 

 sporoplasms initiate the 1st schizogonic phase, characterized by mono- and bi- 

 nucleate schizonts. The 2nd schizogonic phase is characterized by mono-, bl- 

 and quadrinucleate schizonts, by prolific multiplication, by the dense compact 

 nuclei early in this phase, and late in this phase by larger schizonts with less 

 dense vesicular nuclei. This phase terminates in formation of diplokarya. The 

 sporogonic phase is characterized by combination of the 2 nuclei in the diplo- 

 karyon followed by nuclear divisions in a sequence closely resembling meiosis. 

 Two sporoblasts are produced from each sporont. Mature spores in wet mounts by 

 phase contrast were 4.3 ± 0.3 \i long by 2.3 ± 0.2 u wide. The polar filament 

 averaged 76 y long. Mature spores were present about 24 hours after infection. 

 Some observations are presented on an external filament extending from one pole 

 of the spore to host tissue and other events during the proc'.;?s of spore morpho- 

 genesis. 



/ 



