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310 



The neogregarine Mattesla grand is McLaughlin infected larvae and adults of 

 Bracon mellitor Say, a parasite of the boll weevil, Anthonomus grandis 

 Boheman. The li.rvae of B^. mellitor became infected by feeding on infected 

 larvae of the boll woevil, and data suggested that any stage of K. grandis 

 present in the lost was infective. Adults of B^. mellitor became infected by 

 ingesting spores of M. grandis . Healthy adult parasites did not transmit 

 the pathogen to larvae of the boll weevil or to progeny after stinging a 

 diseased boll weevil larva, and diseased adult parasites did not transmit M. 

 grandis to larvae of the boll weevil. Only 1 of 49 progeny of B^. mellitor 

 produced by diseased parents was infected. 



603. ; Andrews, G.; and Bell, M. R. 1971. Field tests for control of 

 Heliothis spp. with a nuclear polyhedrosls virus included in a boll 

 weevil bait. J. Invertebr. Pathol. 18: 304-305. . 

 The test design was too limited to permit evaluation of the bait for control 

 of Heliothis spp.; however, the efficacy of the bait formulations was demon- 

 strated. The bait induced active feeding by larvae; the virus was infective 

 in the formulation; and many larvae became infected in the field. Also, some 

 virus was active in the bait for at least 6 days during hot, dry, sunny weather. 

 We feel that the bait principle should be further investigated as a means of 

 using NPV for effective control of bollwonts. 



604. -■ , and Bell, M. R. 1970. Mass production in vivo of two protozoan 

 pathogens, Mattesla grandis and Glugea gasti , of the boll weevil, 

 Anthonoaaus grandis . J. Invertebr. Pathol. 16: 84-88. 

 A method is described for production in vivo of large amounts of spores of 

 Mattesla grandis and Glugea gasti , both sporozoan pathogens of the boll weevil, 

 Anthonomus grandis . Boll weevil larvae obtained from the mass-rearing section 

 of our laboratory, growing in artificial diet in petri dishes, are inoculated 



