339 



percent of the administered radioactivity was incorporated into the volatile 

 fraction. Also, 4 components of the pheromone comprised 57 to 80 percent of 

 the radioactivity of the volatiles but only 39 percent of the total content of 

 volatiles. Thus, evidence was obtained for d£ novo synthesis of the components. 

 Although the boll weevil is essentially an obligate insect of cotton, this 

 insect does not appear to require an specific component in cotton for bio- 

 synthesis of the pheromone. 



662. ; Lusk, G. J.; and Wiygul, G. 1967. An electrophoretic study of the 

 changes in proteins in the haemolymph during the life cycle of the boll 

 weevil, Anthonomus grandis (Coleoptera: Curculionidae) . Ann. Entoraol. 

 Soc. Am. 60: 1155-1158. 



In electrophoretic study of the proteins in the haemolymph of the boll weevil, 

 Anthonomus grandis Boheman, throughout its life cycle, 14 proteins were found, 

 including one that was unique to the larval stage and one that was unique to 

 the pupal stage. Also, several glycoproteins and lipoproteins were found. 

 Some proteins were common to all stages of the developing insect. However, no 

 definitive correlation could be made between proteins in the egg and in the 

 haemolymph. 



663. ; MauldiYi, J. K. ; and Hedin, P. A. 19bS. Free and protein-bound amino 

 acids in the tissues of the boll weevil, Anthonomus grandis Bokeman 

 (Coleoptera: Curculionidae), during metamorphosis, Corap. Biochem. Physiol. 

 19: 35-43. 



(1) The free and protein-bound amino acids in the pupal tissues during metamor- 

 phosis in the boll weevil, Anthonomus grandis Boheman, were identified and their 

 amounts estimated. (2) Twenty-one amino acids plus ammonia were detected and 

 identified in the extract of free amino acid; hydrolysis revealed 3 more. (3) 

 In the tissue protein 22 amino acids were identified. (4) The total amounts of 



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