^ • 



'.iia^-rsL-rrir ; t^-ja;:!*/; 



■■^^-:rz 



341 



666. , and Wiygul, G. 1970. Effect of y-irradiation on utilization of 

 glycine carbons in biosynthesis of RNA and amino acids in the boll 

 weevil. J. Insect Physiol. 16: 2271-2279. 

 Irradiation of boll weevils, Anthonomus grandis , caused a significant decrease 

 in the amounts of valine, isoleucine, and histidine and an increase in the 

 amount of cystine in the free amino acids but had no quantitative effect on the 

 protein-bound amino acids. Also, in 4 hr it did not affect the incorporation of 

 glycine-1-^ C or glycine-2-^'^C into RNA or the synthesis of the ribonucleic acids, 

 All the carboxyl-labeled carbon of glycine was incorporated into the same posi- 

 tions of both serine and glycine in both the free amino acid and the protein 

 fractions, but some randomization occurred with the a carbon in both irradiated 

 and normal insects. The a carbon of glycine contributed little to the formation 

 of CO-; the carboxyl carbon contributed materially. 



667. , and Wiygul, G. 1971. Synthesis of nucleic acid and protein in the 



boll weevil fed with busulfan. Ann. Entomol. Soc. Am. 64: 822-824. 

 Busulfan (Myleran® (1,4-butanediol dimethanesulfonate)) fed to Anthonomus 

 grandis Boheman (Coleoptera: Curculionidae) inhibited the biosynthesis of 

 DNA and of protein but not the biosynthesis of RNA. Treatment with busulfan 

 did not affect the excretion of C0„ from weevils injected with uridine-2 C 



or thymidine-2C, but it did appear to affect the rate of excretion of CO^ 



14 

 from weevils injected with lysine— C (U) . Sterility in the boll weevil 



therefore results in part from this inhibition of biosynthesis. 



668. , and Wiygul, G. 1972. Contribution of pyrimidines to the biosynthesis 

 of fecal uric acid in normal and busulfan-treated boll weevils. Ann. 

 Entomol. Soc. Am. 65: ,612-613. 



Fecal uric acid from Anthonomus grandis Boheman (Coleoptera: Curculionidae) 



14 



14. 



injected with thymidine-2--^ C and uridine-2--'-^C was radioactive; the guanine 

 present In the feces was not. In normal weevils treated with thymidine, the 



