y 



342 



greatest amount of activity was in the carbon 6 fraction; in similar weevils 

 treated with uridine, the greatest amount was in the carbon 2 and 8 fractions. 

 The pattern of labeling in normal weevils treated with thymidine was changed 

 by f orce-f seding the weevils with busulfan. 



669. , and Wiygul, G. 1973. Uric acid in nucleic and amino acid synthesis 

 In the boll weevil, Anthonomus grandis . J. Insect Physiol. 19: 1569- 

 1574. 



When uric acid-2- C was injected into the boll weevil, Anthonomus grandis , it 



14 

 was metabolized to RNA, DNA, amino acid, and CO2 at the end of 2 hr. The 



free amino acids, lipoamino acids, and protein amino acids were all labelled 



with the free amino acids showing the highest specific activity. Incorporation 



in DNA was slight, but it was extensive in RNA' cytidylic acid showed the 



greatest amount of incorporation. 



670. , and Wiygul, G. 1976. A comparison of certain aspects of nitrogen 

 metabolism in non-diapausing and diapausing boll weevils, Anthonomus 

 grandis . Insect Biochem. 6: 207-209. 



Both DNA and RNA were lower in males and females of a diapausing strain of boll 

 weevils, Anthonomus grandis 10 to 15 days after emergence than in newly-emerged 

 insects of the same strain. Protein content remained relatively constant in the 

 strain of diapausing weevils, but increased substantially in the 10 to 15-day 

 boll weevils of a non-diapausing strain; the relationship of lipoprotein to 

 protein generally remained about the same in both strains for weevils of diffe- 

 rent ages of the same strain. Since the RNA-DNA ratios differed materially only 

 in the 10 to 15-day diapausing males (2.2), it might be used as a corroborative 

 indicator of diapause. The hypothesis that the boll weevils stores nitrogen 

 as uric acid during diapause was disproven; rather the amount of stored uric 

 acid diminished at the onset of diapause. 



