\ 



343 



671. ; Wiygul, G.; and Haynes, J. W. 1977. Inhibition of DNA synthesis in 

 boll weevils ( Anthonomus grandis Boheman) sterilized by Dimllin. 

 Pestic. Biochera. Physiol. 7: 559-563. 



When boll weevils, Anthonomus grandis Boheman, were treated with Dimilin (N-(4- 

 chloropheayl)-N'-(2, 6-difluorobenzoyl)urea) , the biosynthesis of deoxyribonucleic 

 acid was inhibited in the female, but neither ribonucleic acid nor protein synthe- 

 sis was affected. Treated males showed a difference in effect in lipoprotein syn- 

 thesis, whereas no significant difference was demonstrated with females. Testi- 

 cular growth was inhibited in some of the males. Diminishment of sexual function 

 may therefore result in part from inhibition of biosynthesis of DNA by Dimilin. 



672. ; Wiygul, G.; and Lusk, G. J. 1968. Incorporation of lyslne-6-C 

 into the protein of the adult boll weevil, Anthonomus grandis . J. 

 Insect Physiol. 14: 1277-1283. 



The incorporation of lysine-6-C was observed at regular intervals for 24 hr 

 after it was injected into the haemocoele of the boll weevil, Anthonomus grandis 

 Boheman. As the concentration of amino acid diminished in the free amino acid 

 pool, it rose in the protein fraction. However, the concentration in the pool 

 tended to reach equilibrium at a relatively high level after 4 hr, whereas that 

 in the protein fraction levelled off at about 8 hr. The lipoprotein fraction 

 was labelled quickly, within the first hour, and lost its activity as quickly. 

 Probably at least part of the labelled lysine was incorporated into the lipo- 

 protein fraction before it was incorporated into the protein fraction. 



673. ; Wiygul, G.; and Mauldin, J. K. 1968. The free amino acids in the 

 haemolymph of the maturing adult boll weevil, Anthonomus grandis Boheman. 

 Comp. Biochem. Physiol. 25: 139-148. 



1. The free and hydrolyzed free amino acids in the haemol^nnph of the boll 

 weevil, Anthonomus grandis Boheman, were identified, and the amounts present 

 during the first 5 days of adulthood were estaimated. 2. In all, thirty 



\ 



