/ 



345 



reared boll weevils, Anthonomus grandls . It caused significantly greater 

 mortality (88.7 percent) in adult females than in adult males (22.8 percent). 

 The fungus, A. parasiticus , caused very little mortality in either sex of 

 weevils. 



676. ; White; J. R. ; and Bottrell, D. G. 1972. A machine for dispensing 

 grandlure on cigarette filters used to bait boll weevil traps. J. 

 Econ. Entomol. 65: 1215-1216. 



Several formulations of the synthesized pheromone, grandlure, were tested as 

 baits in wing traps placed in the field to attract and capture overwintered 

 and late-season boll weevils. The most promising of these formulations were 

 prepared by dispensing a solution (0.5 ml) of grandlure on a standard cigarette 

 filter (8 X 30 mm). Traps baited with these filters (1/trap) were highly 

 effective in capturing boll weevils in several recent tests (Hardee et al. 

 1972). The design and operation of a labor-saving machine that we constructed 

 to treat filters with grandlure for trapping studies are described herein. 



677. Moore, C. A., and Frazier, J. L. 1976. Partial characterization of acid 

 phosphatases of the boll weevil, Anthonomus grandis . Insect Biochera. 



6: 525-527. 

 Acid phosphatases from boll weevils, Anthonomus grandis have a pH optimum of 

 4.6 and a temperature of 37*'C in 0.09 M citrate buffer. Enzyme activity is 

 inhibited by 0.02 M fluoride (80%), arsenate (67%), and azide (24%), while 

 activation by calcium and magnesium (5%) was considered nonsignificant. 

 Activity of whole body homogenates is greater for females (31.8 Mni/hr/rag 

 protein) than for males (28.3 ym/hr/mg) and varies with adult age. Activity 

 in supernatant and pellet fractions of ovaries was 53.4 and 50.0 um/hr/mg, 

 respectively, while corresponding activity for testes was 68.0 and 60.6 y^,/ 

 hr/mg. 



