385 



were irradiated, mortality did not exceed that of the control. Irradiation 

 over the abdomen and the posterior part of the thorax produced the same level 

 of mortality as did whole-body irradiation. These findings indicate that 

 damage to the midgut is the basic cause of the high mortality among weevils 

 given sterilizing doses of radiation. At 10 days after irradiation, no 

 spermatogonia were found in any testes irradiated with 6000 R or more, and 

 none were found in some testes that received only 2000 R. 



754. Reinecke, L. H.; Klassen, W. ; and Norland, J. F. 1969. Damage to testes 

 and recovery of fertility in boll weevils fed chemosterilants. Ann. 

 Entomol. Soc. Am. 62: 511-525. 

 Studies of mating and histology were made to test the fertility and spermatogenic 

 activity of male Anthonomus grand is Boheman (Coleoptera: Curculionidae) several 

 weeks after the weevils were fed the following chemosterilants: apholate; busulfan 

 (1, 4-butanediol dimethanesu.l fonate) ; 1-aziridinylphosphonic acid cyclic diester 

 with pentaerythritol; D-mannitol 1, 6-dimethanesulfonate; N, N, N*, N'-tetramethyl- 

 P-piperidinophosphonic diamide; and N, N, N', N'-tetramethyl-P-morpholinophosphonic 

 diamide with and without 2 krad of X-rays. Damage to the spermatogenic cycle and 

 the histopathology of testes from treated weevils are described. A'l chemical 

 treatments at 1 of the concentrations used caused permanent damage ♦. j the testes 

 of some weevils as evidenced by the death of all spermatogonia. l>i^ever, none of 

 the treatments caused permanent damage to all the testes at the concentrations 

 used. Damage to the midgut occurred with some chemosterilants. Remating studies 

 showed that recovery of fertility could occur after 4 weeks with 5 chemosterilants, 

 although the data for D-mannltol 1, 6-dimethanesulfonate were insufficient for 

 a firm conclusion. 



