

458 



treated for ( ontrol of Heliothis larvae or boll weevils later in the season. 



899. Tingle, F. C; Lane, H. C; King, E. E.; and Lloyd, E. P' 1971. Influence 

 of nutrients in the adult diet on diapause in the boll weevil. J. Econ. 

 Entomol. 64: 812-814. 



The ratio of proteins and carbohydrates in the adult diet of Anthonomus grandis 

 Boheman was varied to study its effect on the initiation of diapause. Removal 

 of germinated cottonseed puree from the standard diet greatly increased the 

 incidence of diapause, but this affect was offset partially by increasing the 

 level of protein and decreasing the level of sucrose. Similarly, a reduccion 

 of protein and an increase in sucrose caused greater numbers of insects to 

 enter diapause. We were not able to extract the component (s) of germinated 

 cottonseed that prevent initiation of diapause with chloroform, acetone, water, 

 and dilute NaOH, in succession. A cottonseed flour or the alkali-soluble pro- 

 tein of that flour was equal to or better than germinated cottonseed in pre- ""' 

 venting the initiation of diapause. 



900. , and Lloyd, E. P. 1969. Influence of temperature and diet on attain- 

 ment of firm diapause in the boll weevil. J. Econ. Entomol. 62: 596-599. 



When maximum fat and minimum moisture content were used as criteria, adult 

 Anthonomus grandis Boheman fed 11- to 15-day-old bolls and held at 26.7°C 

 attained firm diapause in 1 week; when the temperature was 21.1°C during a 

 10-hr light period and 1C°C during a 14-hr dark period, the weevils took 3-4 

 weeks to attain maximum fat and minimum moisture. Thus, time required for 

 these adult weevils to attain firm diapause was adversely related to tempera- 

 ture. However, the weevils held at 26.7''C and fed 11- to 15-day-old bolls \ 



i 

 attaip^ed firm diapause more rapidly than those fed 1- to 10-day-old bolls ^ 



or squares, and the diet did not influence the time to firm diapause when 

 the weevils were held at the lower temperatures. 



