A92 



taken ci\. 3 weeks to determine that this problem existed by using previously 

 available methods. "• . •,. ' 



973. ; Mitlin, N.; Love, J. N.; and Lusk, G. J. 1970. The absorption and 

 metabolism of glycine-U-"*^ C in the irradiated and normal boll weevil, 

 Antlionomus grandis Boheman (Coleoptera: Curculionidae) . Comp. Biochem. 

 Physiol. 33: 475-480. 



Three-day-old boll weevils, Anthonomus grandis Boheman, that had been irra- 

 diated 2 days previously with 6kR of gamma irradiation were injected with or 



14 

 force-fed glycine-U- C, sampled at intervals for 24 hr and extracted for free 



amino acids, lipoprotein, nucleic acids and protein. Aliquots of the extracts 



were then counted by liquid scintillation procedures. Differences were found 



in the rate of absorption of the glycine from the alimentary canal of irradiated 



and normal insects and in the rate of conversion of free glycine to protein. No 



differences could be detected in the conversion of label to lipoprotein and 



nucleic acids. 



974. ; Mitlin, N.; and Thompson, A. C. 1971. Metabolism of busulfan in the 

 boll weevil ( Anthonomus grandis Boheman) . Pestic. Biochem. Physiol. 



1: 418-423. 

 One-day-old male boll weevils, Anthonomus grandis Boheman, thc.t had been force- 

 fed H, and ^^C-labeled busulfan were held for 24 and 72 hr, respecitvely. The 

 resulting metabolites from the feces and extracts of the weevils were analyzed 

 by thin-layer chromatography, gas-liquid chromatography, and mass spectroscropy. 

 At 72 hr post-ingestion, 34.32% of the total radioc«ctivity was recovered from 

 the feces, about 1.09% as busulfan. Most metabolism of busulfan appeared to 

 take place within 24 hr post-ingestion. Organic acids, amino acids, busulfan, 

 1,4- and 2,3-butanediols, and sulfolane were identified as metabolites. Methane- 

 sulfonic acids was determined as a metabolite by using unlabeled busulfan. 



