[Vou, 2 
822 ANNALS OF THE MISSOURI BOTANICAL GARDEN 
Schramm (’14) ), containing (NH4)2SOx4 as a source of nitro- 
gen, the other with nitrogen supplied as peptone and aspar- 
agin. These media complete were as follows: 
A. B: 
UNC) CES OIC eI IERIE OCH LOFOONoramse Acar ererctsrrler st eee: 10.00 grams 
(NE) SOR reece: .20-grams) ‘Peptone) <2). + teeter 4.00 grams 
Me SO stip oO merit cters vO; grams Aspararingi erect 1.00 grams 
KiSHiP Ornette sree: 210 grams) MegSO727 HeO) vie .10 grams 
LS 0 Vin charmenmta cig bam AM OF trace GIBURO ooogob ene egD0 .10 grams 
Glucosegss-raeeiaeeussrtrete 10/100) grams) HeSOw lace cielo arene trace 
Distilled HsO <......... 500.00 ce. GlucoseWeretreeiarctacraeie 10.00 grams 
Distilled HsOV ee 500.00 ce. 
These media, designated ‘‘A’’ and ‘‘B,’’ were placed in 
125 ec. Hrlenmeyers, about 25 ee. to each flask, and the flasks 
placed horizontally until the agar hardened. A relatively 
large surface was obtained in this way and the harvesting of 
the alga later was facilitated. In inoculation, the alga was 
smeared over the surface of the agar to give an even growth. 
After a growth of thirty days, it was harvested by scraping 
from the agar surface with a stiff, platinum needle. The cells 
were then dehydrated with alcohol, acetone and ether, dried, 
and ground with an equal weight of fine quartz sand. Flasks 
were set up in duplicate in Wollf wash bottles, using 1 per 
cent asparagin as a substrate, with an amount representing 
.oo grams of sand-free algal powder. Checks were run on 
both the asparagin and the algal powder alone. One-half the 
series was taken down at the end of 7 days, the other half at 
the end of 15 days, and the NHg split off determined by the 
Folin method previously employed. The flasks were kept at 
a temperature of 35°C. The results are given in table xvi. 
TABLE XVI 
THE ACTION OF DEHYDRATED CHLAMYDOMONAS CELLS UPON ASPARAGIN 
Suhetrate Weight Nitrogen as NH; in 50 cc. substrate 
50 ce. algal sae ee ee 
1 per cent powder 7 days Net N 15 days Net N 
.35 grams ‘‘A”’ 36 .16 45 mail 
Asparagin .35 grams “B” sl) .96 1.84 1.60 
BA Nr Cae Gols 18 A aes sahsinne 22 fies ote 
Water .35 grams ‘‘A”’ OZ * alist 102i herent 
.35 grams ‘‘B” £03! 9 ieee i 07: Ra ore ee icles 
The amount of nitrogen in the checks is so small as to be 
well within experimental error. The NHzg split off by powder 
