[Vou. 2 
812 ANNALS OF THE MISSOURI BOTANICAL GARDEN 
grams of the tissue powder were used, otherwise the series 
(table x11) was arranged exactly as the preceding and kept 
at room temperature for 25 days. 
Action on other esters——A series was set up with methyl 
acetate, ethyl acetate, and ethyl butyrate in .25 per cent solu- 
tion, using 2 grams of algal powder with 50 ce. of the sub- 
TABLE XIII 
THE ACTION OF POWDERED TISSUE FROM CERTAIN ALGAE UPON TRIACETIN 
Number cc. of N/10 NaOH to neutralize 
10 cc. substrate after 
Alga 10 days 25 days 
Tri Tri Tri 
~ |Water+ . : Water+ t 
eae A tissue nee acidity pcan tissue ae acidity 
OLR coat Aoade ae) .025 sil 175 5 15 SS) 2 
Mesogloea...... 529 .05 all ail 4 ae, aS .05 
Chondrus...... aes .35 sit ail 8 4 aus) as) 
strate in 20 per cent alcohol. Titrations were made from 
time to time against N/10 NaOH with phenylphthalein as an 
indicator. Even after 60 days at room temperature no in- 
crease in acidity was observable over the checks. 
General results for experiments with lipases.—The results 
serve to show that, although slight, there is distinct lipolytic 
activity in most of the forms investigated. The various 
groups of algae are not so distinct regarding this activity as 
was the case with the carbohydrases, nor does the activity of 
the individual alga in this case relate itself particularly to the 
activity shown by the form in its carbohydrase action. Agard- 
hiella hydrolyses the polysaccharides more rapidly than any 
other alga, yet its lipolytic activity is very low. Likewise, 
Laminaria, so inactive in the previous group of enzymes, is 
among the most active on fats. Fucus, on the other hand, was 
found in previous work to have no action on either carbo- 
hydrates or fats. 
The action is especially evidenced by use of the olive oil- 
casein emulsion. In general, the increases were less where the 
aleohol-water emulsion was used—a difference probably ex- 
