1915] 
DAVIS—ENZYME ACTION IN MARINE ALGAE 811 
strate, 5 grams of crushed algal tissue for enzyme action, and 
10 ce. of 95 per cent alcohol as an antiseptic. Checks were 
employed wherein the flasks in one case contained the emul- 
sion alone, and in another case, the same weight of algal pulp 
in distilled water. The flasks were maintained for 15 days 
except for the forms especially noted. At intervals 10 cc. por- 
tions were removed and titrated against N/10 NaOH with 
phenylphthalein as an indicator. 
Inpolytic action on alcohol-water-olive ow emulsion—Be- 
cause of the possibility of the hydrolysis of the casein in the 
emulsion used in the preceding experiments, a series (table 
x11) employing the alcohol-water emulsion was set up as a 
check. This emulsion alone was practically neutral but a 
TABLE XII 
LIPOLYTIC ACTION OF THE SEVERAL ALGAE UPON ALCOHOL-WATER-OLIVE-OIL 
EMULSION 
Number cc. N/10 NaOH to neutralize 10 cc. 
substrate after 10 days 
Alga 
Emulsion | Emulsion Water+ |Water+] Net 
+ tissue alone boiled tissue | tissue | acidity 
Of Resch che ci trciy RRC eT eT 9 .00 .05 .05 85 
EMLErOMOrPnG). 45. ee. + ice 8 .00 .025 sil a 
WAH sea pean doce .65 .00 .05 .075 SOS 
ASCOPNVILUMer a aniston cians or: aD .00 aS ailh .05 
MUTT PETE soa caooncooessoe 1.15 .00 .02 ne .85 
CHONALUS creas josie ecalede tine 1.45 .00 sll .35 1.10 
GU TANS 66. 600b0 00 50M 25 .00 .05 .05 .20 
COUNT oe .85 .00 zi .20 .65 
IRHODVINENTG tense ee ele .525 .00 .05 als .375 
CHOTPI on ee a 5125) .00 5 lS) LS .00 
slight acidity was produced by the addition of the algal 
powder. A negligible amount of the oil globules ran together 
and collected at the surface of the liquid after some days, but 
the bulk of the emulsion stood up well. As in the preceding 
series, 5 grams of the fresh tissue were used as a source of 
lipolytic activity, and the alcohol in which the olive oil had 
been dissolved served as an antiseptic. Fifty cc. of the emul- 
sion were used as a substrate, and the flasks maintained at a 
temperature of 22-23°C. for 10 days. 
Inpolytic action on triacetin—The lipolytic activity of dry 
tissue powder of Ulva, Mesogloea, and Chondrus was tested, 
using a .5 per cent solution of triacetin as a substrate. Two 
