[VoL. 2 
796 ANNALS OF THE MISSOURI BOTANICAL GARDEN 
same ease as starch, seems very slightly acted upon by the 
carbohydrases of Ulva. The failure of action on sucrose and 
lactose is not so surprising as is that on maltose, for one 
would expect the action on polysaccharides to continue to 
what is generally held to be directly assimilable sugars, i. e., 
the hexoses. 
THE CARBOHYDRASES OF ENTEROMORPHA INTESTINALIS 
This series (table 1v) was run under exactly the same con- 
ditions as the one preceding. The ‘‘diffusion-extract’’ was 
from dehydrated tissue about two months old. Ten ce. of this 
were used with each 50 ce. of substrate, toluene added as an 
antiseptic, and the flasks kept at a temperature of 35°C. for 
30 days. 
TABLE IV 
THEACTION OF A “DIFFUSION-EXTRACT" FROM AIR-DRIED ENTEROMORPHA TISSUE 
UPON CERTAIN CARBOHYDRATES 
Sugar as glucose in 5 ce. 
Substrate mgms. 
15 days 30 days 
Starch. sain ccitecean oe 9.7 13.1 
DEXtrin'. . aoc coe 5. 9.8 
Gly covenier. i act ance eerie 2.8 3.9 
NUTTY SevSasos eee orci ere Trace Trace 
SUCIOSG.. 5. 2me fee nis see arte LNereie Trace 
hactose ha aireics mere circ 
Maltose- ern esse ote 
The results for this closely related form are consistent with 
those obtained for Ulva, the action in the present case, how- 
ever, being somewhat slower. The more common polysac- 
charides are acted upon while the disaccharides are not 
attacked. 
THE CARBOHYDRASES OF LAMINARIA AGARDHII 
The water extract from air-dried Laminaria tissue was 
extremely viscous and upon addition of alcohol, a very heavy 
precipitate was thrown down that contained a large amount 
of algal slime. When water was added to this precipitate in 
the usual ratio a very viscous diffusion was obtained. Ten 
ce. of the ‘‘diffusion-extract’’ were used with 50 ce. of the sub- 
strate and 2 per cent toluene added as an antiseptic. The 
flasks were kept at a temperature of 20-22°C. for 100 days, 
