PREMISE FOR CLONE DEFINITION 



SAMPLE CHALLENGES OF 10 CULTURES FROM PLOT FH-if 



1ST PREMISE CHALLENGE: 

 RESULT: 



175 X 176 177 178 180 181 182 183 18^ 185 186 

 NNNYYYNYYN 



REORDER : 

 RESULT : 



180 181 182 18^ 185 176 177 178 183 186 

 YYYYYNNNNN 



2ND PREMISE CHALLENGE 

 RESULT: 



176 X 177 178 183 186 

 N Y Y N 



REORDER 

 RESULT : 



178 183 177 186 

 Y Y N N 



3RD PREMISE CHALLENGE: 177 X 186 

 RESULT: N 



CONCLUSION: k CLONES PROVISIONALLY DEFINED 



FH-41 = 175, 180, 181, 182, 184, 185 



FH-42 = 176, 178, 183 



' 177 



FH-4'+ = 186 



Figure 2— Detailed premise analysis of challenge results 

 obtained from vegetative isolates of the genus Armillaria found 

 on 0.04-ha plot No. 4 located on the Flathead National Forest in 

 Montana (FH-4). 



The blind read was accomplished by working in three- 

 person teams. One constructed the combinations of chal- 

 lenges and labeled (sequentially) each set of six isolates 

 with a challenge number. The isolate numbers and asso- 

 ciated challenge number were recorded, but their plot 

 identification and other data were kept separated. The 

 first round of challenges was accomplished over a period 

 of 12 months, with individual challenges being repeated up 

 to 10 times. These challenges were made in random com- 

 binations among the isolates from within a National 



Forest. Next, Punnett squares were constructed for all the 

 isolates within each plot from within each National Forest. 

 Blind challenges were then constructed to complete pre- 

 liminary clonal definitions (premise-challenge) of the isolate 

 populations from each plot (figs. 2, 3). Further blind 

 challenges were made to complete the verification regions 

 of each Punnett square (fig. 3). The evaluations by the two 

 of us were recorded separately. Thus, each cell of the 

 square could have a mixture of yes and no responses. 



3 



