CHI-SQUARE GOODNESS-OF-F I T TEST FOR CLONAL 

 DELINEATION OF ISOLATES FROM PLOT: FH-4 



PROVISIONAL DEFINITION 



^COUNT NUMBER OF READS IN YES AND NO PORTIONS OF PREMISE LINES 

 ^COUNT NUMBER OF NO READS IN YES PORTIONS 

 ^COUNT NUMBER OF YES READS IN NO PORTIONS 

 >^COMPUTE CHI-SQUARE 



YES REGION NO REGION 



NUMBER EXPECTED 26 60 



NUMBER OBSERVED 2_6 _58_ 



DEVIATION 2 



CHI-SQUARE 0/26 + 4/60 = 0.07 



PROBABILITY OF LARGER CHI-SQUARE (1 df) = 0.50 



^PROVISIONAL DEFINITION OF CLONE DELINEATION ACCEPTED 



VERIFICATION OF CLONE DEFINITIONS 



=<COUNT r-IUMBER OF READS IN VERIFY REGIONS OF PUNNET SQUARE 

 ^COUNT NUMBER OF NO READS IN YES REGIONS 

 =<COUNT NUMBER OF YES READS IN NO REGIONS 

 5<C0MPUTE CHI-SQUARE 



YES REGION NO REGION 



NUMBER EXPECTED 7 4 150 



NUMBER OBSERVED _72_ 148 



DEVIATION 2 8 



CHI-SQUARE 4/72 + 64/150 = 0.48 



PROBABILITY OF A LARGER CHI-SQUARE (1 df) = 0.50 



^PROVISIONAL CLONAL DEFINITION IS VERIFIED 



Figure 4— Explanation of statistical analysis of data 

 obtained from plot FH-4. 



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