Results 



CHROMATOGRAMS 



As might be expected when dealing with a large and complex species such as big 

 sagebrush, considerable chromatographic variation was found. Presently, the plant 

 collections have been divided into two major groups, I and II. Group I has 4 subgroups 

 (la, lb, Ic, and Id). Group II has 2 subgroups (Ila and lib). These groups are based 

 on differences in the chromatographic spots. Chromatograms of each collection always 

 display a basic compliment of ten spots (1, 2, 3, 4, 7, 8, 9, 11, 13, 27) plus varying 

 combinations from an additional eleven spots (5, 6, 10, 12, 14, 16, 22, 25, 26, 33, 36) 

 (table 1 and figs. 1-6). Some of these spots exhibit marked differences in size and 

 intensity of color; therefore, the chromatograms were organized into groups taking into 

 consideration both qualitative and quantitative variations. 



Table 



l.--Rj. values and color of the chromatographic spots in 



Artemisia 



tridentata 





: : Color 







Spot no. 



R 



; f Ultraviolet NH^ + Ultraviolet 



: NH 3 



+ Daylight 



1 



.53/ 



.46 



Blue 



Yellow-green 



Gray 



2 



.90/ 



.78 



Violet 



Violet 





3 



.88/ 



.71 



Blue-green 



Blue-green 





4 



.87/ 



.71 



Violet 



Violet-brown 



Yellow 



5 



• 47/ 



.11 



Yellow or 



Yellow or 











Yel lowish-brown 



Yel lowish-brown 





6 



.28/ 



. 13 



Yellow or 



Yellow or 











Yel lowish-brown 



Yel lowish-brown 





7 



.32/ 



.08 



Violet 



Violet 



Yellow 



8 



.51/ 



.87 



Dark blue 



Dark blue 





9 



.54/ 



.78 



Light iridescent 



Light iridescent 











blue-green 



blue-green 





10 



.18/ 



.84 



Blue 



Blue 





11 



.34/ 



.75 



Blue 



Yellow-green 



Yellow 



12 



.26/ 



.71 





Blue 





13 



.29/ 



.64 



Pink 



Yellow-pink 





14 



.35/ 



.59 



Blue or violet 



Gray-blue or violet 





16 



.32/ 



.90 



Dark blue 



Dark blue 





22 



.32/ 



.84 



Blue 



Blue-green 





25 



.83/ 



.67 



Blue-green 



Blue-green 





26 



.38/ 



.73 



Blue 



Blue 





27 



.51/ 



.57 



Blue 



Yellow-green 



Gray 



33 



.75/ 



.60 



Pink 



Pink 





36 



.26/ 



.84 



Bright blue-green 



Bright blue-green 





3 



