RESULTS 



On the basis of chromatographic variations among collections of big sagebrush, the 

 sources studied are divided into two major groups and seven subgroups (figs. 2-8 and 

 table 1). Several of the chromatographic spots exhibited significant variations in 

 size and intensity of color; so both qualitative and quantitative (table 2) variations 

 were taken into consideration when the chromatograms were organized into groups. 

 Characterization of chromatographic spots of big sagebrush by values and colors 

 (figs. 2-8 and table 2) is nearly identical with data presented in an earlier paper 

 (Hanks and others 1971) . The few changes represent judgments reached after further 

 investigation, except for the R^, value of spot 4, which was misprinted in Hanks and 

 others (1971). The missing numbers in the sequence (table 2) represent spots that 

 appeared only occasionally in the chromatograms and, in most instances, provided no 

 useful basis for the organization of the collections into groups. However, some of 

 these might be indicative of past hybridization between big sagebrush and other 

 sagebrush species . 



Variation within species or subspecies was also observed among other Tridentatae 

 species. Representative chromatograms of each (i.e., A. arhuscula subsp. arbusoula^ 

 A. arhusaula subsp. thermopolae , A. higlaoii , A. cana subsp. bolanderi , A. aana subsp. 

 oana, A. aana subsp. visaidula, A. longitoba , A. nova, A. pygmaea, A. rigida, A. roth- 

 rockii , A. tripartita subsp. tripartita) are illustrated in figures 9-25. Distribu- 

 tion of chromatographic spots among each is included in table 1. R^ values and color 

 variations of the individual spots are given in table 2. 



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