RESULTS 



Proliferations of parenchymatous cells of the pine explants were visible to the 

 unaided eye and easily seen through the polyethylene covers of the culture tubes or 

 through the tube sides. 



Citrus pectin caused the least impact on growth of the pine explants, with all 

 explants showing proliferation in all treatments and only one explant exposed to 

 0.010 precent citrus pectin showing no growth (table 1). Late in the test period, 

 30 percent of the explants in the 0.1 percent treatment became brown, all of them in 

 the 0.5 percent treatment became brown (table 2). 



Table I . --Effects of pectic compounds on in vitro cell profusion of western white 



pine explants 









Concentration 



(percent) 







Treatment 



0.001 



0. 005 



0. 010 



0.025 



0.050 



0. 100 



0.500 



Citrus pectin 



7/7* 



7/7 



6/7 



7/7 



7/7 



7/7 



7/7 



Sodium 

 polypectate 



7/7 



7/7 



7/7 



6/7 



7/7 



6/7 



5/7 



Pectic acid 



7/7 



7/7 



6/7 



7/7 



5/7 



1/7 



0/7 



HOH 



1/7 



0/7 



0/7 



0/7 



0/7 



0/7 



0/7 



*Number of explants showing increase in cell numbers /number of explants treated. 



Table 2 . --Effects of pectic compounds on in vitro color of western white pine explants 



Concentration (percent) 



Treatment 0.001 0.005 0.010 0.025 0.050 0.100 0.500 



Citrus pectin 



7/7* 



7/7 



6/7 



7/7 



7/7 



3/7 



0/7 



Sodium 

 polypectate 



7/7 



7/7 



7/7 



6/7 



7/7 



7/7 



3/7 



Pectic acid 



6/7 



7/7 



5/7 



7/7 



5/7 



0/7 



0/7 



HOH 



1/7** 



0/7 



0/7 



0/7 



0/7 



0/7 



0/7 



*Number of 

 **One callus 



explants retaining a 

 of 49 survived, all 



green 

 others 



color/number o 

 browned in 7- 



f explants 

 10 days. 



treated. 





Sodium polypectate inhibited cell proliferation in 14 percent of the explants at 

 a concentration of 0.1 percent. Inhibition was evident in 29 percent of the 0.5 percent 

 group. Color changes in this test group did not occur until the 0.5 percent level 

 was reached and then 57 percent of the explants became brown. 



2 



