CONCLUSIONS 



The techniques described herein increase the efficiency of culturing a wide variety 

 of conifer tissues. These techniques may also be beneficial to the in vitro establish- 

 ment of many obligate parasites of cultured species. These techniques should not only 

 improve results obtained in culturing conifer species, but may be applicable to the 

 culture of other members of the plant kingdom. 



LITERATURE CITED 



Gautheret , R. J. 



1959. La culture des tissus vegetaux: techniques et realisations. Masson et Cie, 

 Paris. 863 p. 



Harvey, A. E. 



1967. Tissue culture of Pinus monticola on a chemically defined medium. Can. J. 

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, and J. L. Grasham. 



1969a. Procedures and media for obtaining tissue cultures of 12 conifer species. 

 Can. J. Bot. 47: 547-549. 



, and J. L. Grasham. 



1969b. Growth of the rust fungus Cronartium ribiaola in tissue cultures of Pinus 

 monticola. Can. J. Bot. 47: 663-666. 



Koenigs, J. W. 



1968. Culturing the white pine blister rust fungus in callus of western white 

 pine. Phytopathology 58: 46-48. 



Loewenberg, J., and F. Skoog. 



1952. Pine tissue cultures. Physiol. Plant. 5: 33-36. 



Reinert, J. 



1962. Morphogenesis in plant tissue cultures. Endeavour 21: 85-90. 



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