328 Pomona College Journal of Economic Botany. 



In most cultures the lemon yellow oolor appeared first, although in some, 

 yellow and red colors developed almost simultaneously. In cultures thirty 

 to forty days old the red tones became more pronounced than the yellow. 

 A thick pellicle formed ever the surface of the medium, and wherever it 

 touched the gla.ss its color was very striking, being a deep crimson below 

 and shading into the lighter tints of wine red above. 



The potato strains grew only one-half as rapidly as the carnation 

 strains on this medium, and it required about twenty days for a culture 

 to spread over its surface. Strain (VII) developed a dense cottony gro\vth 

 4-6 mm. high. In a direct north light a rose red color began to appear in 

 ten days; in an interior gla.s.s case, the rose red color did not appear until 

 the cultures were thirty days old. No distinctive color developed in cul- 

 tures kept in darknes.s. Strain (II) grew depres.sed or with a cottony growth 

 of a ropy nature. Cultures kept in north light developed a rose red color, 

 but those kept in darkness did not develop any color. 



Steamed white corn meal (M6). The descriptions are given for flask 

 cultures. The carnation strains resemble each other in cultural characters 

 on this medium also. In ten days the surface of the medium became covered 



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with a cottony growth 10 iniu. in height. The usual wine red color began 

 developing in three or four days, which was followed by tlie appearance 

 of the lemon yellow tints. The two colors became mingled throughout the 

 culture, and they gradually increa.sed in intensity with age, until reatihing 

 a maximum intensity in cultures thirty days- old. Numerous rich salmon- 

 colored bodies developed in cultures of that age. A thick wine red pellicle 

 formed over the surface of the medium. In places the mycelium penetrated 

 beneath the pellicle between the glass and the medium, and it is probable 

 that the mycelium is capable of penetrating all through the substratum. 



The potato strains grew less rapidly, requiring twice as much time for 

 a culture to spread over the medium. The growth of strain (VIT) was 

 dense, cottony, and 5-7 mm. high ; no coloration could be detected until 

 the cultures were twenty-five days old, then a faint rose pink color appeared 

 which developed slowly. A thin pellicle formed at the surface of the 

 medium, and no mycelium had penetrated any part of the substratum in 

 cultures sixty days old. The growth of strain (H) was mostly depressed, 

 the little cottony growth that appeared was of a ropy character. A deep 

 lilac color developed from the beginning which later became overspread 

 with a yellowish brown, but if any secondary growth developed the lilac 

 color again appeared. In cultures thirty to forty days old the surface 

 stratum became wTinkled and the mycelium did not penetrate the sub.stratum. 



Steamed rice (M7). The descriptions are given for 200 c. c. flasks. 

 The carnation strains all grew vigorously on this medium, developing a 

 cottony growth 10 mm. high, and particularly delicate colors. Strains (E) 

 arid (R) began developing a lemon yellow color and strain (IV) a wine 

 red color in three days. A few days later all strains developed both colors; 



