March 31, 1905.] 



Facultative parasites or saprophytes. 

 Cells in plates or irregular masses (never 

 in long chains or packets). Acid produc- 

 tion variable. 



Genus 4. Sarcina (Goodsir). 



Saphrophytes or facultative parasites. 

 Division under favorable conditions, iu 

 three planes, producing regular packets. 

 Generally fail to produce acid by fermenta- 

 tion of sugars. 



Genus 5. Ascococcus (Cohn). 



Generally saphrophytic cells imbedded 

 in large irregularly lobed masses of zooglea. 

 In presence of carbohydrates usually form 

 acid. 



Pull paper (preliminarj-) to be published 

 in Science. 



Diagnostic Value of the Bed Color ivhich 

 Develops on the Addition of Caustic Soda 

 to Solutions of Glucose after Fermenta- 

 tion: Wm. R. Copeland and Perkins 

 BoYNTON, Columbus, Ohio. 

 Certain members of the colon group of 

 bacteria produce a substance in glucose so- 

 lutions which, on the addition of caustic 

 soda (NaOH), forms a brick-red color if 

 the alkali is kept in contact with the fer- 

 mented bouillon for twenty-four hours. 



The glucose solution used in making this 

 test contains : 



Meat extract from fresh round beef steak 1,000 c.c. 



Peptone — Witte's best white, dry 10 gms. 



Table salt 5 gnis. 



Anhydrous glucose 10 gnis. 



Reaction (referred to phenol-phthalein) . 1% acid. 



The caustic soda used contains 20 grams 

 of the best grade of NaOH in sticks dis- 

 solved in 1,000 c.c. water. 



The fermentation is carried on by the 

 bacteria for a period of 48 hours at a tem- 

 perature of 37° C. +. The bacillus which 

 brings about the formation of the red color 

 resembles the Bacillus cloacece of Jordan 

 and the Bacillus Zea of Moore. 



487 



The bacterium described by Dr. Theo- 

 bald Smith as the typical 'Bacillus coli 

 communis' forms reactions which differ 

 markedly in every instance from the reac- 

 tions produced by B. cloacece. Therefore, 

 as the colon bacillus never produces the 

 red color in glucose solutions and as B. 

 cloacece does, the appearance of a strong 

 brick red color in a glucose fermentation 

 tube, to which a two per cent. NaOH solu- 

 tion has been added and allowed to digest 

 for 24 hours, may be taken as evidence that 

 the bacteria in the Smith ' tube are B. 

 cloacece and are not B. coli communis. 



I., The Value of the Widal Ileaction for the 

 Diagnosis of Hog Cholera. II., The 

 Production of Agglutinins for Hog 

 Cholera Bacilli in Swine: Chas T. Mc- 

 Clintock, Chas. M. Boxmeyer and J. J. 

 SiFPER, Detroit, Mich. 



1. The serum of normal hogs aggluti- 

 nates strains of ordinary hog cholera 

 bacilli in dilutions occasionally as high as 

 1-250. For this reason we consider a reac- 

 tion in a dilution of less than 1-300 with- 

 out diagnostic value. 



2. The bacillus of swine dysentery is not 

 agglutinated by normal blood in such high 

 dilutions. 



3. The Widal reaction is of no value for 

 the diagnosis of hog cholera, as the disease 

 is at present defined. 



4. The presence of a positive reaction 

 does, however, indicate an infection with 

 cholera bacilli. 



5. There are occasional instances of both 

 natural and artificial infection in which no 

 increase of the agglutinins for hog cholera 

 over those normally present can be dem- 

 onstrated. 



6. The maximum amount of agglutinin 

 develops in a hog's blood within six or 

 seven days after a single inoculation with 

 hog cholera vaccine. 



7. Hogs react to intraperitoneal injec- 



SCIENCE. 



