13() 



in the cells (fig. 64), I dare not deny it decidedly, as it is in reality very difficult 

 to convince oneself of the absence of anastomoses between the chromatophores, 

 which never run quite regularly. According to Kylin (I.e. p. 115) the chromato- 

 phores contain small granules which are interpreted by him as pyrenoids. I have 

 observed the same granules but cannot give any information as to their nature; 

 their appearance seemed not to be constant. While the cells in Lehmann's spec- 

 imens contained fat and no starch, I found in cystocarp-bearing specimens the 

 vegetative cells containing no fat but minute starch-grains staining red-brown in 

 iodine, and the cystocarps, especially the carpospores, contained a great quantity of 

 the same substance. The reaction with iodine was rather similar to that of glycogen. 



The sex-organs develop, as shown by Gran, on 

 special fertile branchlets, generally very near each 

 other. Later, Kylin has accounted for the various 

 combinations of the sex-organs on the same branch- 

 let, but he has not noticed the curious fact that 

 the carpogonia are not always lateral on the fertile 

 branchlet but often intercalary, rising by transfor- 

 mation of the second or even the third cell from 

 the top. The intercalary carpogonia, which were 

 already observed in 1893 by the late Professor 

 Fr. Schmitz who mentioned them in a letter to 

 me, are very common. A very frequent case is 

 represented in fig. 62 A, C, D where the lower cell 

 in a two-celled branch has become a carpogonium, 

 pushing forward a trichogyne from the upper end 

 of the cell along the upper cell which in all cases 

 is sterile bearing two antheridia. In fig. 62 B both 

 the cells have developed into carpogonia, the one 

 superposed on the other. In fig. 62 G the carpo- 

 gonium has arisen from the lowest cell in a three- 

 celled branched branchlet, and in fig. 62 £ and F 

 In the same branchlet a lateral and a terminal carpogonium 

 frequently occur. The intercalary carpogonia show very often a swelling at the 

 base of the trichogyne (fig. 62 D) which may formerly perhaps have been inter- 

 preted as the whole ventral part of the carpogonium. Fertilized carpogonia with 

 adhering globular spermatia frequently occur. After fertilization the separation of 

 the trichogyne takes place in the intercalary carpogonia at the upper end of the 

 swelling (fig. 62 G). Thereafter the fertilized carpogonium increases in length, the 

 trichogyne is pushed aside, and the lengthened body divides by a transverse wall 

 a little under the insertion of the trichogyne (fig. 62 E, F, H). Even in this stage 

 and later the trichogyne with adhering spermatium may yet be visible. In fig. 62 H 

 the primary filament of the young cystocarp is three-celled and has produced a 



Fig, (;2. 



Chantransia ef/loresceiis. Fertile Ijranchlet.s. 

 See the text. I'he eaipogoniu and the cells 

 produced by them alter I'ertilisatlon are 

 shaded, .1— /; IWO : 1, YilHtMl:!. F- // 020:1. 



they are lateral. 



