HABITS OF AMBLYSTOMA 



found much convoluted, indicating a greater degree of flexibility 

 than is the case with stouter spermatozoa like those of Crypto- 



Experinients were performed to determine the length of time 

 the spennatophores would retain their vitality in water, hence the 

 interval within which they would have to be taken up by the female. 

 In all the spermatophores examined the spermatozoa were motion- 

 less; but since the examination was not made until the evening 

 of April 10, probably the spermatophores had been in the water 

 for many hours. The effect of the cloacal secretion of the living 

 female was then tried, to see if it would revive these spermatozoa; 

 no such result was produced. Freshly obtained seminal fluid 

 mounted in water retained its vitality for many hours; but as this 

 experiment was not performed until April 18, only a small amount 

 of seminal fluid could be obtained, and in this the sperms were not 

 m a vigorous condition. It fresh seminal fluid were taken in the 

 proper season and mounted in quantities to correspond with that 

 deposited in a sperniatophore, it might retain its vitalitv much 

 longer. I'he viscous lupiid in which the spermatozoa occur does 



V fHsliK ,]q>„s,u,l sp(rnuitophoie ot J nfon um/oa//s ^^as 



tlu bill n\ .,,(,!.,,!.. . . uti. t. i..d .| .If in.l (xun! I 1 hi 

 tl.(> n.icn.s.-niK-. K\rvvu Unuvs fn.i.i th. tiui, tlu .[x nnatopliore 



tophore thui ^n^\n^ ^^nuU\ In Ktiuud l..n.( i tli ui r^^(l^< lioui 



C. The Eggs. Those of A. punrfafiim have hvvu dcscnlu'd 

 and hgurul In ( laik (SO) Uu < uith th<n iii.hM.h ,1 

 gelatinous envelopes, occur m conipact buii( li(>s, MirioiiiHU'd hv 

 axerx tlmkidh mass Thetntiu Miiutint i> u.ii ,lh ot w omI 

 sh.ipt <)tltnn«ail\ is laigt n.u . h t Hu .>t J f ,/> nam 



ai( , ii.h lu^.i ih... lh(.M ..t I In/, mum ind du nninh . ot 

 cgn-s „, a bmich IS ii.iiallv n-iva1.T. 



Vt th( tiiiu ot i\u <I.M< XMN ot du .pu.n.tnphoH ^n^ U^^ 

 biMKhont (ould In tuuu.l Ih. iiini.lKi uhK iih k , <d 



for a week; at the eiitl ot that tune eggs were iound in early seg- 



