The artificial media (table 2), media preparation, rearing containers, 

 and techniques used in this study were similar to those reported by Gal- 

 ford (1967 and 1969^). The media had been pressed to a moisture con- 

 tent of 50 to 55 percent. Holes just large enough to accommodate the 

 larvae were made in the media with a dissecting needle, and the larvae 

 were transferred with a small brush. 



Scolytid species were reared to adults in the glass plate rearing devices, 

 while larvae of the cerambycid species were started in the glass plate rear- 

 ing devices and transferred to 60 x 15-mm plastic petri dishes full of 

 unpressed media at the end of 3 to 4 weeks. The ceramycid larvae were 

 transferred to fresh media at monthly intervals until they pupated. All 

 rearing was conducted in darkened cabinets at 30±2°C and 50±20 per- 

 cent relative humidity. 



1 Galford, Jimmy R. A larval and ovipositional medium for scolytus multistriatus. 

 (Accepted for publication in the J. Econ. Entomol.) 



Table 2.— Artificial media formulas 



Constituent 



Medium A 



Medium B 





Grams 



Grams 



Agar 



32.00 



32 



Sucrose 



16.00 





Fructose 



8.00 





Glucose 



8.00 





Vitamin diet fortification mixture 



12.00 





Brewer's yeast^ 



40.00 





Soybean protein 



16.00 





Wesson's salt mixture 



20.00 





Cholesterol 



.80 





Kretschmer wheat germ^ 



20.00 



120 



Vegetable lecithin 



.80 





Vitamin Bt 



.08 





Sorbic acid 



2.00 



2.00 



Methylparaben 



1.00 



1.00 



Alphacel (hydrolyzed) ^ 



320.00 



280 





ml. 



ml. 



Wheat germ oil 



4.00 





Water 



800.00 



800.00 



1 Mention of specific brand products does not imply their endorsement by 

 the USDA. 



4 



