99 



Action of Coleus. 



On February 13, '02, I carried Coleus from the University- 

 greenhouse to the greenhouse of the Department of Botany, The 

 plants had been grown where a day temperature of 66 degrees F, 

 was maintained. Although the temperature was 6 degrees F, below 

 freezing, the plants experienced no change of temperature in the 

 transfer, because of the double box in which they were carried. 

 The temperature was taken before and after the trip by the same 

 thermometer. Most of the pots were placed on a greenhouse bench. 

 Three were carefully taken from pots with dirt undisturbed and 

 placed in wide mouthed bottles filled with water, and, as the 

 dirt soaked, it fell to the bottom of the bottle and left the 

 roots free in the water. Twelve hours later they were greatly 

 wilted. Two were placed under bell-.iars, with constantly satura- 

 ted atmosphere, to prevent transpiration: the other on table in 

 the greenhouse: wilting continued and leaves fell from lower 

 part of stem, February 18/02 still much wilted, A fresh plant 

 was then taken and its roots, dirt, pot and all immersed in water 

 to its lowest leaves: In thirty six hours the plant was much 

 wilted so that the lower leaves fell off. The plant continued 

 in this wilted condition. Another one was gradually (during three 

 days) watered until finally immersed to first leaves. Wilted in 

 24 hrs. after immersion: lower leaves fell: continued wilted 



