LACTATE DEHYDROGENASE IN THE RETINA OF THE GUPPY 



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The purpose of the following investigations was: i) to establish with starch 

 ;el electrophoresis at what developmental stage of the embryo or postembryo 

 >f Lebistes the E-bands appear; 2) to détermine with kryostat sections in what 

 ayers of the retina they réside and then to identify their subcellular location; 



+ 



Fie. 2. 



Effect of inhibitor (3 M urea) on lactate dehydrogenase isozyme pattern of adult Lebistes eye. 

 Starch gel plate sliced horizontally : upper half incubated without, lower half with urea. 



3) to follow the development of the E-isozymes both with starch gel electrophoresis 

 and kryostat sections until sexual maturity is reached. 



Results 



The first problem was to show the E-isozymes selectively. To that effect 

 several known inhibitors for mammalian LDH isozymes were tested. The only one 

 that had an effect on starch gel, as well as on kryostat sections, was urea in a 

 3 M concentration (fig. 2). 



